The nucleolar detention pathway

Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stress

Nucleolar proteins play an important role in the regulation of the MDM2-p53 pathway, which coordinates cellular response to stress. However, the mechanism underlying this regulation remains poorly understood. Here, we report that the nucleolar protein CSIG is a novel and crucial regulator of the MDM2-p53 pathway. We demonstrate that CSIG translocates from the nucleolus to the nucleoplasm in res...

TPL2-NPM-p53 pathway monitors nucleolar stress

Serving as the cellular factory for the biogenesis of ribosomes (the molecular machines responsible for the decoding of mRNAs to proteins), the nucleolus controls a vast array of physiological processes including cell growth and proliferation. It thus comes as no surprise that inherited and acquired abnormalities in ribosome biogenesis can lead to tumorigenesis and that changes in size and numb...

Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes

Eukaryotic 60S ribosomal subunits are comprised of three rRNAs and ∼50 ribosomal proteins. The initial steps of their formation take place in the nucleolus, but, owing to a lack of structural information, this process is poorly understood. Using cryo-EM, we solved structures of early 60S biogenesis intermediates at 3.3 Å to 4.5 Å resolution, thereby providing insights into their sequential fold...

Novel nucleolar pathway connecting intracellular energy status with p53 activation.

In response to a shortage of intracellular energy, mammalian cells reduce energy consumption and induce cell cycle arrest, both of which contribute to cell survival. Here we report that a novel nucleolar pathway involving the energy-dependent nucleolar silencing complex (eNoSC) and Myb-binding protein 1a (MYBBP1A) is implicated in these processes. Namely, in response to glucose starvation, eNoS...

Detention Centres—The First Phase